New Classes of Conditional Toxins as Therapeutic Agents Against Breast Cancer
Final rept. 1 Apr 1998-31 Mar 2001
CALIFORNIA INST OF TECH PASADENA
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During the three-year duration of this grant DAMD17-98-1-8042, the Idea Grant, we focused on the following projects 1 Development of signal-regulated, cleavage-mediated toxins. During this time, we completed the work on the CUP9-mediated, HIV protease-dependent conditional toxins. A paper describing these results has been finished its completion was delayed by difficulties in some of the necessary control experiments. This paper will be submitted for publication in the Fall of 2001. 2 We also carried out, during the first year of support, an extensive study whose major aim was to construct better portable degrons degradation signals that can be used, in particular, for designing conditional toxins. This effort was entirely successful. A paper describing these results was published at the end of 1999 Suzuki Varshavsky. Degradation signals in the lysine-asparagine sequence space. EMBO J. 186017-6026,1999. A reprint of the paper is enclosed. 3 In 1999, we also completed the construction of a bivalent inhibitor of the N-end rule pathway, a useful reagent for conditional-toxin projects, and published this new approach Kwon Varshavsky. Bivalent inhibitor of the N-end rule pathway. J. Biol. Chem. 27418135-18139, 1999. 4 Other projects that encompass the subject of this grant have also been carried out. They were less successful, given a variety of technical difficulties that we encountered, at the Idea-Grant stage of the work.
- Medicine and Medical Research