Establish an In Vitro Model for the Study of NF2 Gene Function and Gene Therapy
Annual rept. 1 Oct 1999-30 Sep 2000
HOUSE EAR INST LOS ANGELES CA
Pagination or Media Count:
Mutation analyses of the NF2 gene from NF2 patients suggest that the inactivation of the NF2 gene, and the consequent lack of gene product protein known as MerlinSchwannomin is the primary cause of this disease. Within the three years period, we plan to develop a laboratory protocol to establish Schwann cell culture using surgical specimens from NF2 patients, and compare the tumor cells from patients with different NF2 gene mutations. In addition, we want to extend the life span of primary culture cells by immortalizing them using a retrovirus which we engineered. This will not only ensure the reproducibility of results within the NF2 community, but also provide scientists greater access to certain materials for the study of biologic function of Schwann cell and also important for testing therapeutic approaches. During the first 12 months, an additional ii primary cultures were added to the 55 previously banked NF2 tumor cultures. Two primary human vestibular schwannoma cultures from different NF2 patients and one normal Schwann cell culture are in the process of immortalization.
- Medicine and Medical Research