Accession Number:

ADA394233

Title:

Cathepsins B and K: Role in Metastasis of Human Prostate Cancer to Human Bone in an In Vivo SCID-hu Mouse Model

Descriptive Note:

Annual rept. 15 Feb 2000-14 Feb 2001

Corporate Author:

WAYNE STATE UNIV DETROIT MI

Personal Author(s):

Report Date:

2001-03-01

Pagination or Media Count:

30.0

Abstract:

Cathepsins K and B have been implicated in bone resorption in several pathologies. Expression of cathepsin B is highly upregulated in human prostate cancer. Cathepsin K, in contrast to cathepsin B, is expressed predominantly in osteoclasts. Cathepsin K has recently been identified in tumor cells within human breast tumor bone metastases and in adjacent osteoclasts, suggesting that cathepsin K may participate in bone resorption by tumor cells. Therefore, we are testing the hypothesis that cathepsins B and K are responsible for osteolysis by human prostate cancers. One goal in this first year was to establish stable GFP-expressing transfectants of the three prostate cancer cell lines in order to facilitate their detection by confocal laser fluorescence microscopy both in vitro and in vivo. This will also allow us to more readily assess the interaction of the prostate cancer cells with the surrounding tissue and bone matrix. Another goal was to establish selective assays for determining expression of cathepsins B and K at the transcript, protein and activity levels and for following changes in localization of the two enzymes by immunofluorescence staining in vitro and in situ as well as by analysis of secretion in vitro.

Subject Categories:

  • Medicine and Medical Research

Distribution Statement:

APPROVED FOR PUBLIC RELEASE