Synthetic Lethality in Breast Cancer Cells: Genes Required for Tumor Survival
Annual summary rept.1 Jun 2000-1 Jun 2001
COLD SPRINGS HARBOR LAB NY
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The conversion of a normal cell into a cancer cell proceeds through a series of genetic and epigenetic alterations. We have proposed to use well-established genetic methodologies to identify novel anti-cancer targets via their specific, genetic interactions with common cancer mutations. In short, we will identify genetic alterations that are neutral in normal cells, but that are lethal when combined with cancer mutations. This synthetic lethality approach may identify potential therapeutic targets that are highly specific to the cancer cell. In the past year, we have made substantial progress toward the goal of developing technologies necessary to making this type of target search a reality. We have devised genetically defined human cancer models that can be used to conduct synthetic lethality screens and have developed new methodologies for manipulating gene expression in mammalian cells. This work has resulting in two manuscripts that are now under review for publication.
- Genetic Engineering and Molecular Biology
- Medicine and Medical Research