Omega-3 Fatty Acids and a Novel Mammary Derived Growth Inhibitor Fatty Acid Binding Protein MRG in Suppression of Mammary Tumor
Annual rept. 1 Jul 2000-30 Jun 2001
LONG ISLAND JEWISH-HILLSIDE MEDICAL CENTER NEW HYDE PARK NY
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We have previously identified and characterized a novel tumor growth inhibitor and a fatty acid binding protein in human mammary gland and named it as Mammary derived growth inhibitor Related Gene MRG. MRG has tumor-suppressing activities it inhibits breast cancer cell growth in vitro and tumor growth in vivo. Here, the effects of MRG on mammary gland differentiation and its interaction with omega-3 polyunsaturated fatty acids omega-3 PUFA on growth inhibition were investigated. MRG protein expression was associated with human mammary gland differentiation with the highest expression observed in the differentiated alveolar mammary epithelial cells from the lactating gland. Overexpression of MRG in human breast cancer cells induced differentiation with changes in cellular morphology and a significant increase in the production of lipid droplets. Treatment of mouse mammary gland in organ culture with MRG protein resulted in a differentiated morphology and stimulation of beta-casein expression. Treatment of human breast cancer cells with 3 PUFA DHA resulted in a differential growth inhibition proportional to their MRG expression.
- Medicine and Medical Research