Role of Tissue Transglutaminases in Breast Cancer
Final rept. 1 Jul 1997-30 Jun 2000
DUKE UNIV MEDICAL CENTER DURHAM NC
Pagination or Media Count:
Angiogenesis is vital to tumor growth and metastasis. The scope of this grant was to study in detail the role of Tissue transglutaminase tTG during wound healing and tumorigenesis. In the first year of the grant proposal, we have described the expression, localization, molecular form and tTGs association with other major determinants of wound healing and tumorigenesis. Our findings clearly show that tTG is readily upregulated in wound healing and rat mammary adenocarcinoma and is associated with endothelial and inflammatory cells. Hypoxia, Vascular endothelial growth factor, Transforming growth factor beta and Tumor necrosis factor alpha are also upregulated alongside tTG in those cells. tTG is quickly proteolysed in the tissues and that may have important consequences as tTC can hydrolyze ATPGTP in its fragmented form. In the second year and continuing, We have also shown that tTG can inhibit tumor growth as part of host response mechanism and identified that tTGs effects are compartmentalized i.e. host and tumor. In tumor cells, it prevents growth and metastasis at primary site and promotes establishment of metastasis once the tumor cells escape from the primary focus. We are still continuing with establishing the mechanism behind tTG effects.
- Anatomy and Physiology
- Medicine and Medical Research