Nutritional Effect on Androgen-Response Gene Expression and Prostate Tumor Growth
Final rept. 1 Aug 1998-31 Jan 2001
NORTHWESTERN UNIV EVANSTON IL
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The objective of our research is to understand the molecular mechanism underlying the impact of various dietary components including fat, genistein, vitamin D, and selenium on prostate growth. Our research has demonstrated that the ventral prostate of the rats on high fat diet is 15 pO.OO1 heavier than that of the rats on low fat diet. The dietary influence on ventral prostate weight does not seem to involve androgen action axis because dietary components did not influence the expression of several androgen-response genes, serum testosterone T and dihydrotestosterone DHT, and intraprostatic T and DHT in experimental animals. Thus, high fat diet is likely to modulate the ventral prostate weight via an androgen-independent mechanism. Using a highly sensitive PCR-based cDNA subtraction method, we have identified one gene that is down-regulated by dietary fat in the ventral prostate. Sequencing analysis revealed that this gene encodes prostatein C3, a ventral prostate-specific secretory protein. Dietary fat down-regulates the expression of prostatein C3 by 37 pO.O5. Prostatein C3 represents the first example of genes that are regulated by dietary fat in the prostate in vivo. Our findings are potentially important because dietary fat is a major risk factor associated with prostate cancer incidence.
- Anatomy and Physiology
- Medicine and Medical Research
- Food, Food Service and Nutrition