The Preclinical Evaluation of Fever-Range, Whole Body Hyperthermia as an Adjuvant to Chemotherapy and Cytokine Immunotherapy for the Treatment of Breast Cancer
Annual summary rept. 1 Jun 2000-31 May 2001
HEALTH RESEARCH INC BUFFALO NY
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This predoctoral grant was written to examine the effect of combining fever-range whole body hyperthermia with cytokine immunotherapy and chemotherapy in animal models of breast cancer. Progress in year number two has recently improved with the implementation of a new animal model that closely represents human breast cancer and will replace the less appropriate CT26 model a colon cancer model used in year number one. This represents the most significant advance. The 4T1 cell line is a murine mammary adenocarcinoma syngenic to balbc mice that spontaneously metastasizes. Although only preliminary experiments have been performed suggesting that IL-l2 decreases lung metastasis, the model is now solidly in place in the laboratory. Secondly, the work with the CT26 model has moved forward slightly and the importance of IFN-gamma to the observed enhancement of the anti-tumor response with the combination of IL-l2 and whole body hyperthermia noted in year one has been hypothesized. Unfortunately, a bizarre, septic shock-like syndrome has been observed that has hampered further progress in both animal models the past 4 months. The etiology of this syndrome is still under investigation by the PI and the Roswell Park Cancer Institute Department of Laboratory Animal veterinary staff.
- Anatomy and Physiology
- Medicine and Medical Research