Cloning and Characterization of Active Egr-1 Target Genes by In Vivo Crosslinking
Annual summary rept. 1 May 2000-30 Apr 2001
BURNHAM INST LA JOLLA CA
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The purpose of this project is to identify the profile of Egr-l target genes in breast cells. Normal breast cells are shown to express Egr-l, while breast cancer cells do not. It is, therefore, important to identify the nature of those target genes regulated by Egr- 1 which are absent in breast cancer cells. I have approached this goal by performing in vivo crosslinking of Egr-l to its target sites in breast cells, followed by immunocapture of Egr-l together with its targets. In this report I have proceeded with the identification, by multiplex PCR amplification, of Egr-l target genes. Specifically, I have successfully cloned a novel full length cDNA and describe anew Egr-l target gene called TEXl. Expression of TEXl has the biological activity of growth inhibition in a cell cycle dependent manner. I am proceeding to further characterize this new gene as well as identifying additional Egr-l target genes expressed in both normal and breast cancer cells.
- Genetic Engineering and Molecular Biology
- Medicine and Medical Research