Accession Number:

ADA390407

Title:

Growth Inhibition of Breast Tumor Cells by Hypodense and Normodense Eosinophilic Cell Lines

Descriptive Note:

Annual rept. 15 Jun 1999-14 Jun 2000

Corporate Author:

HOWARD UNIV WASHINGTON DC

Personal Author(s):

Report Date:

2000-07-01

Pagination or Media Count:

47.0

Abstract:

These studies were based on the hypothesis that activated eosinophils release substances mediators that inhibit the growth of breast tumor cells in vitro. Cultured supernatants 24hr, 48hr and 72hr from eosinophil cell lines were examined for growth inhibition of MCF-7 and MDA-MB- 231 breast tumor cell colony formation. Serial 2-fold dilutions 12, 14 and 18 inhibited both MCF-7 and MDA-MB-23 1 colony formation in vitro. The time of harvesting of the supernatants affected their inhibitory capacity 72hr 48hr 24hr. Prior testing of eosinophil conditioned media revealed the presence of IL-4, and current examination revealed the presence of TNF alpha4Opgml. When exogenous cytokines were used, TNF alpha potently inhibited colony formation of MCF-7, while IL-3, IL-4, IL-S were marginal in their activity 10-35 inhibitory activity.

Subject Categories:

  • Anatomy and Physiology
  • Medicine and Medical Research

Distribution Statement:

APPROVED FOR PUBLIC RELEASE