Inhibiting Tumorigenesis by Growth Factor Receptor Down Regulation Using a Sorting Nexin
Annual rept. 15 Apr 1999-14 Mar 2000
ARKANSAS UNIV FOR MEDICAL SCIENCES LITTLE ROCK
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Excessive activation of growth factor receptors can lead to the unrestrained cellular proliferation characteristic of tumors. Our objective is to determine if SNXl, a protein involved in intracellular membrane trafficking, can be used to downregulate EGF receptors in mammary gland. Our approach is to characterize the gene for SNXl and to generate transgenic animals overexpressing SNXl in mammary glands. We have characterized a genomic clone for SNXl and had planned to use this clone for transgenic vector construction. However, the size of the first intron in SNXl is too large for this approach to be used successfully. Instead, a WAP-SNXl cDNA vector has been constructed and we are now ready to generate transgenic animals. A Career Development Award was a second component of the application. Career development activities include participation as reviewer on the American Cancer Society Cell Structure and Metastasis study section and participation on a search committee charged with identifying a Director for Breast Cancer Research at UAMS. In addition, the State of Arkansas Breast Cancer Research Program awarded me a one-year pilot research grant to examine the relationship between HER-2Theu and EGF receptors in mammary gland cell proliferation.
- Medicine and Medical Research