Accession Number:

ADA386850

Title:

Isolation of Motile Tumor Cells from Live Breast Tumors

Descriptive Note:

Annual rept. 1 Jun 1999-31 May 2000

Corporate Author:

ALBERT EINSTEIN COLL OF MEDICINE BRONX NY

Personal Author(s):

Report Date:

2000-06-01

Pagination or Media Count:

38.0

Abstract:

During metastasis from a primary tumor, cell motility is believed to be important for dissemination of tumor cells from the primary. An understanding of the mechanisms of cell motility in metastatic tumor cells in vivo, in particular, would be important for a more rational system of diagnosis and treatment of metastatic cancers. We have developed a new method for imaging tumor cells in live animals and have identified subpopulations of moving cells within primary breast tumors. Previous to this approved application, we described only a metastatic cell-line MTLn3 using this technique. Since then, we have described a non-metastatic cell line MTC and reported the observable differences between the two cell lines. We proposed to use this novel imaging method to collect the motile subpopulation of tumor cells in live primary tumors which can then be used for further analysis to define how these cells differ from their non-motile neighbors in the same tumor and to define how cell motility contributes to metastasis. We have been able to differentially collect the motile sub-population of metastatic cells and shown that due to the differing chemotactic response to EGF, we were able to selectively collected the metastatic cell

Subject Categories:

  • Anatomy and Physiology
  • Medicine and Medical Research

Distribution Statement:

APPROVED FOR PUBLIC RELEASE