Accession Number:

ADA386846

Title:

Immunotherapy of Breast With Tumor RNA Transfected Dendritic Cell Vaccines

Descriptive Note:

Annual rept. 1 Sep 1999-31 Aug 2000

Corporate Author:

DUKE UNIV MEDICAL CENTER DURHAM NC

Personal Author(s):

Report Date:

2000-09-01

Pagination or Media Count:

11.0

Abstract:

The major research focus of this grant is to develop effective treatments for patients with low volume metastatic disease using dendritic cell DC -based vaccines loaded with tumor RNA. Toward this goal, during the second year of this grant we have made the following progress 1 Improved efficiency of RNA transfection into tumor cells using electroporation. 2 Improve methods to isolate and amplify tumor RNA from a small number of tumor cells. 3 . Develop methods for isolating breast tumor cells from the blood or bone marrow of breast cancer patients. We have continued to encounter difficulties in identifying patients with sufficient tumor cells to initiate the clinical trial with non- amplified RNA Specific aim 2 . Fortunately, development of the RNA amplification methodology has progressed faster than we anticipated in the grant application and it now appears that we will be poised to carry out the trial using amplified RNA Specific aim 4, in year 3 rather than year 4 and complete the trial including analysis during the life of this grant. We are very pleased of the prospect of exceeding our time line as shown in the Statement of Work

Subject Categories:

  • Anatomy and Physiology
  • Medicine and Medical Research

Distribution Statement:

APPROVED FOR PUBLIC RELEASE