Accession Number:

ADA386839

Title:

Analysis of the TACC1 Gene from the 8p11 Chromosome Region Frequently Amplified in Metastasizing Breast Cancer

Descriptive Note:

Annual rept. 1 Jun 1999-31 May 2000

Corporate Author:

CLEVELAND CLINIC FOUNDATION OH

Personal Author(s):

Report Date:

2000-06-01

Pagination or Media Count:

6.0

Abstract:

The TACC1 gene was isolated from a region of chromosome 8pll which shows amplification in breast cancers, especially those which show metastasis to the lymph nodes. Preliminary studies showed that overexpression of TACC1 in mouse fibroblasts causes morphological transformation. We have now shown, using immunohistochemistry and immunoprecipitation that TACC1 is present in the cytoplasm and the nucleus and furthermore that it probably associates with the mitotic spindle microtubules. Yeast two hybrid analysis has identified a series of genes which interact with TACC1. Two of these, SIAH1 and GAS4l, appear to be associated with growth regulatory pathways which may implicate TACC1 in cell growth control and hence why overexpression leads to progression of breast tumors. Two other proteins, p16 and L-Sm7, also interact with TACC1 and these proteins are apparently involved in the transportation of proteins to the nucleus. Thus it appears that TACC1 makes important associations in the cytoplasm which then facilitate the transportation of TACC1 and probably the whole protein complex into the nucleus where it may affect mitosis. Further characterization of the intracellular pathways which use TACC1 for their function will help further understand its role in breast tumorigenesis.

Subject Categories:

  • Anatomy and Physiology
  • Medicine and Medical Research

Distribution Statement:

APPROVED FOR PUBLIC RELEASE