Regulatory Pathways Involved in Heregulin-Induced Proliferation and Differentiation of Human Breast Cancer Cells
Annual summary rept. 6 Apr 1999-5 Apr 2000
IOWA UNIV IOWA CITY
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The polypeptide heregulin has been shown to reverse the transformed phenotype of certain cultured breast cancer cell lines and hence convert them to more normal, milk-producing cells. In contrast, heregulin is reported to induce proliferation of other breast cancer cell lines. These diverse effects of heregulin are mediated by receptors in the ErbHER family, which function as coreceptor complexes. How the different signaling pathways activated by ErbB family receptors can elicit alternatively the differentiation or proliferation of breast cancer cells was investigated in the previous year of funding. Our experiments addressed the mechanism of activation of mitogen-activated protein kinase MAPK by hergulin coreceptors. As one read-out of MAPK activation, we monitored the nuclear translocation of the activated enzyme by confocal immunofluorescence microscopy.
- Anatomy and Physiology
- Medicine and Medical Research