Accession Number:

ADA386759

Title:

Determining Gene Expression, Substrate Specificity and Natural Substrates for Prostate Associated Proteases

Descriptive Note:

Annual rept. 1 Jul 1999-30 Jun 2000

Corporate Author:

CALIFORNIA UNIV SAN FRANCISCO

Personal Author(s):

Report Date:

2000-07-01

Pagination or Media Count:

48.0

Abstract:

We work on serine proteases of the chymotrypsin fold due to the wealth of information that exists on structure-function relationships regarding this class of enzymes and the existence of potent and specific inhibitors that are readily available for their inhibition. In addition, understanding of the function of these proteases may lead to further insight into cancer biology and may lead to possible therapeutics. We have focused upon an enzyme known as membrane-type serine protease 1 MT-SPlthat has been implicated in prostate cancer. During our first funding year, we have determined the in vitro cleavage substrate specificity of the enzyme using positional-scanning combinatorial libraries and substrate phage display. The preferred cleavage sequences were found to be P4-ArgLys P3-X P2-Ser Pl-Arg Pl-Ala and P4-X P3-ArgLys P2-Ser Pi Arg Pi Ala where X is a non-basic amino acid. We have also identified two macromolecular substrates, single-chain urokinase-type plasminogen activator sc-uPA and protease activated receptor 2 PAR2 . The affinity of MT-SPl for these key extracellular targets suggests an important role in pathological and regulatory events such as tumor cell invasion and metastasis.

Subject Categories:

  • Biochemistry
  • Anatomy and Physiology
  • Medicine and Medical Research

Distribution Statement:

APPROVED FOR PUBLIC RELEASE