Response of Breast Cancer Cells to Hormonal Therapy; Quantitative in Vivo NMR Studies
Annual rept. 15 Aug 1998-14 Aug 1999
CALIFORNIA UNIV BERKELEY
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Our research is directed at determining the specific metabolic pathways and the individual reactions affected by estrogen and tamoxifen in both estrogen receptor-positive ER and estrogen receptor-negative ER- breast cancer cells through the use of novel NMR methodology and quantitative analysis. In this report period, we have quantified the effect of estrogen on metabolic fluxes in ER cells. This was accomplished using a comprehensive metabolic model and NMR data from cell extracts. The model was further refined to allow for the incorporation of isotopomer, or multiply-labeled, metabolite data. Microcarrier culture, flask, and roller bottle studies were continued in order to expand the methodology used to quantify breast cancer cell metabolism under varied treatment conditions. In addition, investigations were performed in flasks to characterize the dose response of cells to the metabolic inhibitor cerulenin.
- Medicine and Medical Research