Study the Pathogenic Role of erbB-3, erbB-4 and their Ligand Heregulin in Human Breast Cancer Cells
Final rept. 1 Jul 1996-30 Jun 2000
GEORGETOWN UNIV WASHINGTON DC
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Among the growth factor receptors, members of the class I receptor tyrosine kinase family ErbB is most frequently implicated in human breast cancers. In the last report, we delineate the biological function of ErbB-4 by down-regulation of ErbB-4 expression in breast cancer cell lines with specific ErbB-4 ribozymes. To further elucidate the significance of ErbB-4 in breast cancer, we investigated the expression of ErbB-4 in primary breast carcinoma by utilizing immunohistochemical analysis with an anti-ErbB-4 monoclonal antibody on a breast cancer tissue microarray. ErbB-4 expression was found in 60 344 of 571 of the 571 samples examined. High intense immunoreactivity of ErbB-4 was detected in 13 74 of 571 of these primary breast tumors. Most of the staining was found in both cell membrane and cytoplasm. No expression was detected in majority of benign tumors.
- Medicine and Medical Research