Functional Analysis of Breast Cancer Susceptibility Gene BRCA2
Annual rept. 11 Jul 1998-11 Jul 1999
MOUNT SINAI SCHOOL OF MEDICINE NEW YORK
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Two monoclonal antibodies against different regions of BRCA2 mAb B 1 and mAb B3 were characterized. Both of them specifically recognized wild type BRCA2, and mAb B 1 can also specifically recognize C-terminal truncated BRCA2 protein in Capan-1 cell. I detected the interaction between BRCA2 and 3 Rad51-related proteins which are involved in DNA repair and demonstrated that BRCA2 coexists in biochemical complex with hsDMC1, a human meiosis- specific RecA homologue, but not with XRCC2, Rad51D or the replication Protein RPA. The specific interaction of BRCA2 and hsDMCl suggests that BRCA2 may be involved in DNA recombination and repair both in germ and somatic cells. I have involved in a new BRCA2 associated protein, BRAF35, and demonstrated that 1 it is a nuclear protein. 2 it interacts specifically with BRCA2 in mammalian cells. Turns of the functional relationship between BRCA2 and BRAF35 is under investigation.
- Anatomy and Physiology
- Medicine and Medical Research