Applications of a Novel Nucleic Acid Detection Method in Breast Cancer Analysis of Overexpression of HER-2/neu and FAK
Annual rept. 1 Jul 1999-30 Jun 2000
NORTH CAROLINA UNIV AT CHAPEL HILL
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The routine, low-cost detection of genetic material is an important step in translating the advances in cancer genetics to therapeutics and diagnosis. In this study, metal oxide electrodes and a solution oxidant of DNA have been used to detect electrochemically the products of the polymerase chain reaction PCR performed on niRNA from the HER-2 gene in BT-474 cancer cells. This method can be used to detect as little as 500 attomoles of DNA quickly and at low cost. The technique has recently been expanded to include detection of the size of PCR fragments, which is reflected in the size of the measured currents. This technique has been applied to sequences that are particularly difficult to size and amplify, such as trinucleotide repeats.
- Genetic Engineering and Molecular Biology
- Anatomy and Physiology