Human Adrenal Androgens: Regulation of Biosynthesis and Role in Estrogen-Responsive Breast Cancer in a Mouse Model
Final rept. 1 Sep 1994-31 Aug 1999
BAYLOR COLL OF MEDICINE HOUSTON TX
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These experiments investigate a mouse model for the biosynthesis of the human adrenal androgens dehydroepiandrosterone, DHEA, and its sulfate, DHEAS and the role of these steroids in human breast cancer growth. An androgen-dependent human breast cancer model was established in immunodeficient scid mice. Zona reticularis cells in the human adrenal cortex are responsible for adrenal androgen biosynthesis because of the suppressed expression of 3 beta-hydroxysteroid dehydrogenase 3 beta-HSD in these cells. A protein present in the non-DHEA-secreting zones of the cortex and absent from the zona reticularis which binds to a regulatory region of the type II 3 beta-HSD gene was partially purified. Human adrenocortical cells were transplanted into scid mice and were shown to replace the animals own adrenal function.
- Medicine and Medical Research