Ultramicro Methods in Bochemistry. III. The Determination of Serum Cholesterol. IV. The Determination of Glucose
Technical Progress rept.
LOVELACE FOUNDATION FOR MEDICAL EDUCATION AND RESEARCH ALBUQUERQUE NM
Pagination or Media Count:
Ultramicro procedures for the determination of serum cholesterol and of serum, plasma, or whole blood glucose are described. The chromogenic reaction of cholesterol with ferric iron was adapted to an ultramicro method. The results obtained with this procedure are compared to those obtained with a macro analysis which applies the chromogenic reaction of cholesterol with acetic anhydride and sulfuric acid in the presence of p-toluene sulfonic acid. Statistical evaluations of the method to determine reproducibility and variance are presented. The reliability of this methodin the presence of elevated serum bilirubin has been verified. One determination done in duplicate requires 20 microlitersl of serum. The color reaction obtained when glucose reacts with Beta-glucose oxidase in the presence of peroxidase and o-anisidine was evaluated for ultramicro analysis. The results obtained are compared with those given by the Technicon AutoAnalyzer procedure which utilizes the reduction of ferricyanide by glucose. Statistical evaluation of the precision of the method and variance analysis are presented, and the precautions necessary for reliable determination of glucose are emphasized. One determination done in duplicate requires 10 microloters of whole blood, serum, or plasma.
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