Tumor Targeting with Phage Library
Annual summary rept. 1 Jul 1999-30 Jun 2000
BURNHAM INST LA JOLLA CA
Pagination or Media Count:
I have proposed to identify peptides that bind to the vasculature of prostate cancers by using a technique developed in our laboratory called in vivo phage display, and then to use these peptides to isolate their vasculature-specific receptors. Specific markers for the vasculature of prostate cancers can be good targets for anti-prostate cancer drugs and will lead to a greater understand- ing of prostate carcinogenesis. I have also taken an alternative approach to identify prostate cancer vasculature-specific markers by directly comparing the mRNA profiles between endothelial cells from prostate tumors and normal organs. For this project I have accomplished the following Established an expression cloning strategy using phage-displayed RGD4C peptide and avp3-expressing HEK cells as a model system. Established three ecdysone-inducible R-Ras cell lines for testing cDNA microarray and GeneChip technologies. Used sequential in vitro transcription to setup a highly sensitive gene-profile assay that is 10,000 times more sensitive than the original method. Isolated highly pure endothelial cell populations from various organs and prostate tumors of Tie-2LacZ tansgenic mice.
- Genetic Engineering and Molecular Biology
- Medicine and Medical Research