Accession Number:

ADA383701

Title:

Tumor Targeting with Phage Library

Descriptive Note:

Annual summary rept. 1 Jul 1999-30 Jun 2000

Corporate Author:

BURNHAM INST LA JOLLA CA

Personal Author(s):

Report Date:

2000-07-01

Pagination or Media Count:

7.0

Abstract:

I have proposed to identify peptides that bind to the vasculature of prostate cancers by using a technique developed in our laboratory called in vivo phage display, and then to use these peptides to isolate their vasculature-specific receptors. Specific markers for the vasculature of prostate cancers can be good targets for anti-prostate cancer drugs and will lead to a greater understand- ing of prostate carcinogenesis. I have also taken an alternative approach to identify prostate cancer vasculature-specific markers by directly comparing the mRNA profiles between endothelial cells from prostate tumors and normal organs. For this project I have accomplished the following Established an expression cloning strategy using phage-displayed RGD4C peptide and avp3-expressing HEK cells as a model system. Established three ecdysone-inducible R-Ras cell lines for testing cDNA microarray and GeneChip technologies. Used sequential in vitro transcription to setup a highly sensitive gene-profile assay that is 10,000 times more sensitive than the original method. Isolated highly pure endothelial cell populations from various organs and prostate tumors of Tie-2LacZ tansgenic mice.

Subject Categories:

  • Biochemistry
  • Genetic Engineering and Molecular Biology
  • Medicine and Medical Research

Distribution Statement:

APPROVED FOR PUBLIC RELEASE