The Identification of Genes Differentially Regulated by Progestins and Glucocorticords in Human Breast Cancer Cells
Annual summary rept. 1 Jul 1999-30 Jun 2000
COLORADO UNIV HEALTH SCIENCES CENTER DENVER
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Progestins and glucocorticoids are two classes of steroid hormone with very distinct biological functions. Progestins have been shown to be related to the incidence and progression of breast cancer, but glucocorticoids have no direct relation with breast cancer to our knowledge. However, progesterone receptor and glucocorticoid receptor share many structural and functional similarities including identical DNA sequence recognition characteristics. In order to understand the role of progestins in breast cancer and how progesterone receptor mediates this hormone specific regulation, we proposed to identify genes that are differentially regulated by progestins and glucocorticoids in the human breast cancer cell line T47DAl-2 using two systematic strategies a retroviral promoter- trapping strategy and an oligonucleotide array analysis. A modified retroviral promoter-trapping system has been established. Several clonal cell lines that represent potential hormone differentially regulated genes were generated using this system. In the Affymetrix oligonucleotide array analysis, genes that are differentially regulated by progestins and glucocorticoids were identified. The findings from these studies provide us interesting study candidates for understanding the mechanisms of gene differential regulation by progestins and glucocorticoids in breast cancer as well as how progestins function specifically in breast.
- Genetic Engineering and Molecular Biology
- Medicine and Medical Research