Parmagnetic Oligonucleotides for Breast Cancer Imaging
Annual rept. 1 Jul 1999-30 Jun 2000
OHIO STATE UNIV RESEARCH FOUNDATION COLUMBUS
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Our hypothesis is that novel paramagnetic oligonucleotide analogs will be useful MR contrast agents for breast cancer imaging. This novel approach of targeting MR contrast agents will ultimately aid in the early detection of metastatic breast cancer. The technical objectives are as follows along with progress to date. 1 Optimization of Paramagnetic Oligonucleotide Chelates To prepare a series of paramagnetic chelate modified oligonucleotides to assess maximal proton relaxation enhancement upon binding to a macromolecular target. The oligonucleotide conjugates have been prepared and are ready to assay. 2 Selection of RNA Aptamer for the Tn-antigen To use the SELEX technology to find a small RNA sequence which binds specifically to the tumor associated Tn-antigen. The SELEX process has been initiated. 3 Evaluation of Paramagnetic Oligonucleotides in Breast Cancer Cell Model System To use a cell culture model system for evaluating the proton relaxation enhancement of paramagnetic oligonucleotide analogs which have been targeted to breast cancer cells. A significant amount of work has been done to develop and evaluate the novel monolayer assay system. We are now ready to examine our agents using this system.
- Medicine and Medical Research
- Radiation and Nuclear Chemistry