Diagnosing Breast Cancer Using Protease Fingerprint
Annual summary rept. 1 Jun 1999-31 May 2000
BURNHAM INST LA JOLLA CA
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I constructed a substrate phage display library displaying randomized hexamer amino acid sequences to explore the possibility of profiling protease activity in non-metastatic breast tumor and metastatic breast tumor. This technique was first applied to two breast cancer implicated and closely related metalloproteinase, MMP-2 and MMP-9. Novel substrate specificity was found for MMP-2 vs. MMP-9. These novel substrate sequences can serve as a tool for us to investigate the role of MMP-2 and MMP-9 during breast tumor progression. Moreover these unique substrate sequences can be used as leads for designing specific metalloproteinase inhibitors to intervene or eradicate breast tumor. Most importantly, the success of defining substrate specificity of MMP-2 and MMP-9 validates the feasibility of profiling protease activity via substrate phage display library technique.
- Medicine and Medical Research