Development of Integrase-Like Catalytic Antibodies for Breast Cancer
Final rept. 1 Sep 1997-31 Oct 1999
TEXAS UNIV MEDICAL BRANCH AT GALVESTON
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This project aims to develop catalytic antibodies that will allow efficient DNA transfer by promoting DNA site-specific recombination. Such antibodies may provide a very powerful means to manipulate DNA transfer at defined sequences with obvious implications for gene therapy of breast cancer. In order to engineer enzymes that catalyze the recombination, we immunized mice with a synthetic DNA Holiday structure and screened over 5,000 hybridomas. We had candidate clones that seemed to either resolve or bind to the structure in primary screenings. Unfortunately we have not been able to isolate clones that possess the properties after expansion and purification. For constructing fab expression library we encountered difficulties in amplifying Fab cDNA from immunized mouse splenic mRNA. We have made a few primary libraries. We are in the beginning of screening the libraries for the Fabs that resolve the structure. This is a very technical and labor challenging project. I feel that our resources are limited to successfully complete the project. Successful completion of this project will provide a new technology of DNA transfer for both biomedical research and therapeutic purposes.
- Anatomy and Physiology
- Medicine and Medical Research