Tumor Suppressor Genes in Early Breast Cancer and its Progression
Annual rept. 1 Sep 1998-31 Aug 1999
WASHINGTON UNIV ST LOUIS MO SCHOOL OF MEDICINE
Pagination or Media Count:
Ductal carcinoma in situ DCIS is a pre-invasive stage of breast cancer in which the malignant cells have not penetrated the basement membrane. From our allelotyping study of DCIS, chromosomal regions of 8p, 13q, l6q, 17p and 17q showed loss of heterozygosity LOR significantly above background 5. In our previous report we described the LOR mapping of a region on 8p 30 LOR containing a putative tumor suppressor gene TSG which we refined to a small interval of 1.4 cM between the markers D8S265 to D8SD520. Last year 1998 we completed the construction of a fine structure radiation hybrid map and an integrated YACBAC contig spanning the 1.1 -cM deletion region. During the past project year, we mapped eleven expressed sequence tags ESTs including 4 previously reported ESTs to this contig. Twenty-three overlapping oligo overgo probes were generated using end sequences derived from CITB BAC clones. These overgos were used to screen RPCI-l 1 BAC library. A total of 299 clones including CITB BAC clones were isolated and then fingerprinted. A comprehensive sequence-ready contig that provides a useful resource for sequencing the critical region has been assembled using clones with good fingerprinting data.
- Anatomy and Physiology
- Medicine and Medical Research