Tumor Associated Antigenic Peptides in Prostate Cancer
Annual rept. 30 Sep 1998-29 Sep 1999
NEW YORK MEDICAL COLL VALHALLA
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The objective of this proposal was to identify and isolate novel prostate cancer antigens. We proposed to identify these novel antigens in an experimental rat model using purified preparations of the heat shock protein gp96 and a library of synthetic distinct antibodies that were available in the combinatorial phage display library. We demonstrated the tissue specificity in tumor rejection properties of purified preparations of gp96 by utilizing purified preparations of tumor and non-tumor gp96 preparations. By the use of a library of phage display antibodies we differentially selected out tumor reactive phages. These experiments substantiated our hypothesis that indeed these antibody libraries serve a useful tool to identify tumor specific immunogenic peptides. The specific antibody carrying phages and soluble antibodies produced from these phages were then used to identify novel tumor associated cellular protein.
- Medicine and Medical Research