Analysis of the Role of Cortactin in Tumor Cell Invasion
Annual rept. 1 Jul 1998-30 Jun 1999
AMERICAN RED CROSS ROCKVILLE MD
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Breast cancer is frequently associated with gene amplification at the chromosome 11 q 13. Studies have demonstrated that cortactin also EMS1, a filamentous actin F-actin associated protein and a substrate of protein tyrosine kinase Src, plays an important role in the amplification. However, the pathological role of cortactin in breast cancer is unknown. We have developed a form of cortactin mutant that is deficient in tyrosine phosphorylation and is no longer to be regulated by Src. The mutant and wild-type forms of cortactin and oncogenic Src were introduced into MDA-MB-231 cells, a highly invasive breast cancer cell line. Overexpression of wild-type cortactin or oncogenic Src potentiate the motility of the cells, while overexpression of the mutant form impairs the cell migration as well as cell invasion into extracellular matrix. This study indicates that cortactin may play an important role in metastasis associated with breast cancer.
- Genetic Engineering and Molecular Biology
- Medicine and Medical Research