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Accession Number:
ADA379615
Title:
Training in Support of Research Project Entitled "Tumor Cell De-adhesion by Aberrant, Single Skubmit Integrins".
Descriptive Note:
Annual rept. 1 Jul 98-30 Jun 99
Corporate Author:
BURNHAM INST LA JOLLA CA
Report Date:
1999-06-01
Pagination or Media Count:
6.0
Abstract:
My purpose is try to identify a plasma membrane protein fingerprint which can be used to distinguish invasive breast cancer cell lines from non-invasive breast cancer lines. The methods included those for purification of plasma membrane proteins , extraction of proteins using ureathiourea mixture, a standard procedure for 2D PAGE, excision of polypeptide spots from gels, trypsin digestion, MALDI TOF mass spectrometry and database searching for protein identity are well-established for this project. Three breast cancer cell lines MDA-MB435, MDA-MB23 1 and MCF7 with different metastatic potential have been grown and 2-D maps of plasma membrane proteins have been generated in the laboratory. Computer- assisted analysis of the 2D polypeptide pattern showed that 56 of the polypeptide spots were the same between MCF7 and MDA-MB23 1 two non-metastatic Breast Cancer cell lines, only 42 of the polypeptide spots were the same between MCF7 and MDA-MB435 metastatic vs non-metastatic Breast Cancer cell lines. This differential expression of proteins is being characterized in order to determine which proteins are important to the metastatic condition. A number of proteins from the 2D gels has been identified by MALDI TOF mass spectrometry. Recently, antibody library phage display, has been expanded for further identifying the plasma membrane proteins.
Distribution Statement:
APPROVED FOR PUBLIC RELEASE
