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Drug Development and Conservation of Biodiversity in West and Central Africa.
Annual rept. 1 May 99-30 Apr 00
PACE UNIV NEW YORK NY
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This research was conducted as part of Associated Project 3 of the International Cooperative Biodiversity Group ICBO program awarded to the Walter Reed Army Institute for Research WRAIR. This project concerns the detection of growth inhibition of African trypanosomes and of pathogenic trichomonads by phyto- extracts from West Africa. During this period, 21 extracts received from WRAIR were screened against one Trypanosoma brucei strain and three Trypanosoma rhodesiense strains. Eight of these had IC50 values of 0.1 to 20 ugml, and were of interest for further studies. Four extracts from previous studies were tested in vivo in a L brucei mouse model, at up to 50 mgkgday i.p. x 3 days. None prolonged the life-span of infected animals. Additionally, based on trypanosome sterol requirements and evidence of plant sterols in active plant extracts, five anti-hypercholesteremic agents used in clinical medicine were tested in the trypanosome mouse model. None prolonged the life-span of infected animals at up to 100 mgkgday for 3 days. Sixteen extracts were tested vs. metronidazole-sensitive and -resistant isolates of Trichomonas vaginalis and a Tritrichomonasfoeus isolate. Of these, five had MIC values of 0.1 mgml including an extract of Dracaena mannii which gave MIC values of 0.0125 to 0.006 mgml.These studies are continuing, with increased emphasis on in vivo testing of more highly purified plant extracts in an effort to determine the active agents in these extracts.
APPROVED FOR PUBLIC RELEASE