Accession Number:

ADA379225

Title:

Mosaic Landscape Phage as a New Class of Detector

Descriptive Note:

Final rept. 1 Jul 1998- 31 Jan 2000

Corporate Author:

MISSOURI UNIV-COLUMBIA

Personal Author(s):

• Petrenko, Valery A.
• Smith, George P.

Report Date:

2000-04-19

Pagination or Media Count:

8.0

Abstract:

Detection of single molecules or particles of biological or organic agents requires a ligand, which could be immobilized to a chip of analytical platform and should provide strong specific binding of the agent and generation of a detectable signal. We suggested new ligands-landscape phages selected from landscape libraries or mosaic landscape libraries. By testing landscape libraries with a panel of representative antigens streptavidin from the bacterium Streptomyces avidinii, avidin from chicken egg white, and b-galactosidase from Escherichia coli we obtained a proof of conception of using landscape phage as a new type of ligand for detector platforms. A library of organic landscapes is a rich source of substitute antibodies-filaments that can bind protein and glycoprotein antigens with nanomolar affinities and high specificity.

Descriptors:

• *ANTIBODIES
• DETECTORS
• BACTERIA
• PEPTIDES
• PROTEINS
• GLYCOPROTEINS
• CHIPS(ELECTRONICS)
• STREPTOMYCES
• ESCHERICHIA COLI
• LIGANDS
• MOSAICS(DETECTORS)
• LIBRARIES
• ANTIGENS
• IMMUNOGLOBULINS
• BACTERIOPHAGES

Subject Categories:

• Biochemistry

Distribution Statement:

APPROVED FOR PUBLIC RELEASE