Accession Number:

ADA379162

Title:

Development of a Reproductive Toxicity Test Using Xenopus.

Descriptive Note:

Final rept. 8 Sep 97-15 May 00

Corporate Author:

OKLAHOMA STATE UNIV STILLWATER

Personal Author(s):

• Fort, Douglas J.
• Bantle, John A.

Report Date:

2000-05-01

Pagination or Media Count:

111.0

Abstract:

Current reproductive toxicity tests may be missing significant toxicity by not examining the gametes of both male and female organisms. The purpose of the present study was to develop and validate a reproductive toxicity test using Xenopus laevis which measures effects on gametogenesis, reproductive performance, and development fitness of the progeny. Sexually mature female and male Xenopus laevis were exposed to varying concentrations of cadmium chloride Cd, boric acid B, or ethylene glycol monomethyl ether EGME, in order to examine the effects on gametogenesis and reproductive outcome. Frogs were exposed for a period of 30 days and then either bred to untreated malesfemales or their gonads were examined to determine effect on gametogenesis. Results from these studies indicated that each toxicant was capable of inducing adverse reproductive effects including gametogenesis, reproductive performance and development effects in a concentration-dependent manner. Preliminary evaluation of the protocol for reproductive assessment appear favorable.

Descriptors:

• *TOXICITY
• *REPRODUCTION(PHYSIOLOGY)
• *GERM CELLS
• *SEX GLANDS
• TEST AND EVALUATION
• METHYL RADICALS
• TEST METHODS
• ADVERSE CONDITIONS
• CHLORIDES
• CADMIUM
• ETHERS
• MONOMERS
• MALES
• FROGS
• FEMALES
• BORIC ACID

Subject Categories:

• Anatomy and Physiology
• Medicine and Medical Research
• Toxicology

Distribution Statement:

APPROVED FOR PUBLIC RELEASE