Role of the ATM Gene in Bilateral Breast Cancer Following Radiotherapy.
Annual rept. 11 Jul 98-10 Jul 99
MOUNT SINAI SCHOOL OF MEDICINE NEW YORK
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The purpose of this project is to determine whether women who developed bilateral breast cancer following radiotherapy for an initial breast cancer had a higher incidence of heterozygosity for a mutation in the A TM gene than either breast cancer patients who did not develop a second breast cancer or bilateral breast cancer patients who did not receive radiotherapy. The approach has been to amplify with PCR the ATM gene in segments employing either cDNA produced using RNA isolated from blood lymphocytes or DNA extracted from paraffin-embedded biopsy tissue samples. During the second year of this project, primers were designed and validated to amplify all exons that comprise the ATM gene. In addition, DNA was isolated from 41 bilateral breast cancer patients and exons 2, 3, 4, 1 5 and 24 were screened for mutations using a Non-radioisotopic RNase Cleavage- based Assay NIRCA. No mutations were discovered in these exons from any of the bilateral breast cancer patients. It is planned that over the course of the third year of this project, a mutational analysis will be performed for all remaining ATM exons in the bilateral breast cancer patients under study.
- Anatomy and Physiology
- Medicine and Medical Research