Accession Number:

ADA374168

Title:

Role of mp 170 Seprase in Breast Cancer.

Descriptive Note:

Annual rept. 20 Jun 98-19 Jun 99

Corporate Author:

GEORGETOWN UNIV WASHINGTON DC

Personal Author(s):

Report Date:

1999-07-01

Pagination or Media Count:

19.0

Abstract:

SepraseFAPalpha, a cell surface serine-type gelatinase with Mr 170, is thought to be involved in tumor invasion and metastasis. However, I have shown previously that overexpression of SepraseFAPalpha in human SW-13 and MCF-7 epithelial carcinoma cells, has no effect on their Matrigel invasion, matrix adhesion and proliferation properties. Mammalian, bacteria and baculovirus expression systems have all been used to express and purify the molecule for further characterization, however, with little success. Rihozyme constructs targeting SepraseFAPalpha were made, and despite their in vitro activities, none of which was effective in depleting endogenous levels of SepraseFAPalpha in 1205LU and MDA-MB-436 cells. During the course of this project, a novel secreted fibroblast growth factor binding protein FGF-BP2 was also characterized. I have demonstrated that FGF-BP2 has tumor promoting activity in both in vitro and in vivo. Whereas, FGF-BP2 is absent in normal breast tissues, abundant messages were induced in some breast tumor tissues, and most melanoma samples. Thus, in contrast to the apparent bystander role of SepraseFAPalpha, preliminary evidences suggest that FGF-BP2 may play a role in cancer growth and metastasis.

Subject Categories:

  • Anatomy and Physiology
  • Medicine and Medical Research

Distribution Statement:

APPROVED FOR PUBLIC RELEASE