Protein Markers of Chemical Exposure and Molecular Epidemiology
Final technical rept. 1 Apr 96-31 Mar 99
INDIANA UNIV-PURDUE UNIV AT COLUMBUS DEPT OF BIOLOGY
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This final technical report describes the results of experiments that 1 explored, at the molecular level, the toxicity of lead in various exposure systems 2 enabled the development a system of general and tissue-specific protein markers for lead in rabbits and rats that might be indicative of its effect in human toxicologic targets 3 assessed the cellular expression of GST isoenzymes as protein markers of lead toxicity in rat kidney and 4 investigated the suitability of tissue-slice in vitro toxicologic approaches by evaluating their patterns of protein expression and comparing them to patterns from whole tissues. High resolution large scale two-dimensional polyacrylamide gel electrophoresis 2-DE with computerized image analysis was used to map celltissue proteins of various lead targets. In the case of lead experiments, proteomics technology, e.g. mass spectrometric analysis of tryptic digests peptide-mass fingerprinting of selected proteins resolved by 2-DE , was used to identify altered proteins. Quantitative and qualitative assessments of toxic effect were made, individual protein data was compiled in annotated databases, and protein markers identified.
- Medicine and Medical Research