Accession Number:

ADA371830

Title:

Cellular Analysis of Circadian Rhythmicity in Cultured SCN Neutrons

Descriptive Note:

Final rept. 1 Feb 97-30 Jun 99

Corporate Author:

MASSACHUSETTS GENERAL HOSPITAL BOSTON

Personal Author(s):

Report Date:

1999-12-07

Pagination or Media Count:

4.0

Abstract:

Within the mammalian hypothalamus, the suprachiasmatic nucleus SCN contains a circadian clock for timing of diverse neuronal, endocrine, and behavioral rhythms. We have tested the hypothesis that the circadian period in behavior expressed by the whole animal is determined by the collective period that arises from the coupling of a large population of clock cells with diverse circadian periods. The results clearly show that circadian period in the whole animal is determined by averaging widely dispersed periods of individual clock cells. We have also shown the inhibitory transmitter GABA can phase-shift individual clock cells in culture. A phaseresponse curve to GABA has been generated for individual clock cells, and we have shown that daily GABA pulses can synchronize clock cells.

Subject Categories:

  • Anatomy and Physiology

Distribution Statement:

APPROVED FOR PUBLIC RELEASE