Accession Number:

ADA371647

Title:

A Hand-Held Evanescent Wave Multianalyte Biosensor with Fully Disposable Components

Descriptive Note:

Final rept. 1 May-31 Oct 99

Corporate Author:

RESEARCH INTERNATIONAL INC WOODINVILLE WA

Personal Author(s):

Report Date:

1999-12-16

Pagination or Media Count:

4.0

Abstract:

Phase 1s primary objective was a handheld, multi-analyte, wet-chemistry instrument design that could measure a wide range of chemicals, proteins, viruses, bacteria and biological fragments at concentrations ranging to below one part per billion. This was to be accomplished through an integration of state-of-the-art digital and analog circuitry with novel miniaturized fluid handling technologies, optics, and highly specific antibody-based assay techniques. Successful implementation of a dot-style immunoassay strategy was demonstrated on a prototype molded polystyrene optical element. Experimental tests strongly substantiated the design strategy. A 5-channel electro-optic module was constructed that produced signal levels well within the measurement ability of state-of-the-art PIN photodiode technology, and experimental binding curves for model assays were remarkably smooth, lacking in noise, and exhibited no optical cross-talk between channels.

Subject Categories:

  • Microbiology
  • Optical Detection and Detectors

Distribution Statement:

APPROVED FOR PUBLIC RELEASE