Accession Number:

ADA371153

Title:

Molecular Mechanisms of Metastasis Suppression in Human Breast Cancer.

Descriptive Note:

Annual rept. 1 Jul 98-30 Jun 99,

Corporate Author:

PENNSYLVANIA STATE UNIV COLL OF MEDICINE HERSHEY

Personal Author(s):

Report Date:

1999-07-01

Pagination or Media Count:

152.0

Abstract:

The major cause of cancer deaths can be attributed to metastasis. Our goal was to identify metastasis-controlling genes for human breast cancer. This research is based upon our finding that microcell-mediated transfer of chromosome 11 into MDA-MB-435 results in nearly complete suppression of metastasis without suppressing tumorigenicity. The key findings during this reporting period were 1 identification of three novel genes which are more highly expressed in metastasis-suppressed human breast carcinoma cell lines 2 demonstration that one of the novel genes, designated BrMS1 Breast Metastasis Suppressor-1, suppresses breast cancer metastasis in a dose-dependent manner 3 BrMS1 maps to 11q13, a site commonly altered in late-stage breast carcinoma 4 Preliminary results include 1 Full-length for one of the two other novel cDNAs has been cloned. Transfections have been initiated and colonies are beginning to appear. Despite several attempts, we have been unsuccessful in obtaining full-length for the third novel gene. In short, we have essentially completed the major objective of this proposal and are completing initial characterization of the candidate metastasis suppressor gene.

Subject Categories:

  • Genetic Engineering and Molecular Biology
  • Medicine and Medical Research

Distribution Statement:

APPROVED FOR PUBLIC RELEASE