Accession Number:

ADA371075

Title:

Detection of DNA Adducts in Human Breast Tissue.

Descriptive Note:

Final rept. 1 Jun 96-30 Jun 99

Corporate Author:

NORTHEASTERN UNIV BOSTON MA

Personal Author(s):

Report Date:

1999-07-01

Pagination or Media Count:

45.0

Abstract:

Cancer of the breast and other tissues may be initiated or promoted by DNA adducts. Some of these adducts may be unknown at the present time, and differ from one individual to another both qualitatively and quantitatively. This makes it important to detect the entire spectrum of DNA adducts in breast and other tissues. Analytical methodology is not available for this at the present time, and there are practical or fundamental barriers against current methodology ever acquiring this capability. In this project new analytical methodology which promises to fill this gap has undergone significant development. The new methodology is based on fluorescence-dye postlabeling of DNA adducts, followed by capillary electrophoresis with laser-induced fluorescence detection. A more stable, second generation dye reagent was prepared and ultrapurified immobilized metal ion affinity chromatography was set up to conveniently remove the dye after the labeling step the methodology was applied in a preliminary way to the analysis of biological samples including human tissues and it was found that the dye-labeled DNA adducts are readily detected by matrix assisted laser desorption ionization mass spectrometry.

Subject Categories:

  • Anatomy and Physiology
  • Medicine and Medical Research

Distribution Statement:

APPROVED FOR PUBLIC RELEASE