Identification of Protein Components of Yeast Telomerase.
Annual rept. 1 Sep 97-31 Aug 98
PRINCETON UNIV NJ
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Telomeres are specialized tandemly repeated DNA structures found at the natural ends of most eukaryotic chromosomes. They are required both for protecting chromosome from degradation and replicating the ends of chromosomes. Most eukaryotes replicate telomere by a special reverse transcriptase called telomerase. Another pathway of telomere formation is telomere-telomere recombination that uses homologs either in telomeric or subtelomeric repeats as the substrates. I am working on two projects involving Saccharomyces cerevisiae telomere formations. S. cerevisiae replicate their telomeres by using the telomerase pathway. In the first project I focus on understanding the nature of the S. cerevisiae telomerase. I found the Est2p reverse transcriptase and RNA subunit TLCl form a 2OS telomerase complex in vivo. In the second project I tagged the subtelomere Y element with a marker to study telomere- telomere recombination. I found that telomerase minus cells can survive through telomere-telomere recombination using either TG-rich or Y sequences as substrates.
- Medicine and Medical Research