Identification of Retinoid Induced Growth Suppressing Genes
Annual rept. 1 Sep 97-31 Aug 98
COLUMBIA UNIV NEW YORK
Pagination or Media Count:
Year 1 has uncovered unexpected difficulty in infecting T47-D cells with recombinant retroviruses. Possible explanations are discussed above and will be addressed prior to proceeding, since the enhancer trap strategy requires efficient infection. Two additional points will be considered. First, de novo methylation of provirus might not compromise the enhancer trap strategy since it is the flanking cellular genes that provide the transcriptional elements in this system. Second, it is possible to essentially multiply infect entire cell populations by co-culturing with mitomycin C treated producing cell lines for 3-5 days. Therefore I do not feel that this approach need be abandoned yet. Initially, I predicted that the constellation of interesting genes induced by retinoic acid in T47-D cells would include 1 phosphoprotein phosphatases,2 GTPase activating proteins,3 protein kinases,4 transcriptional repressors. As documented in Cho et al., 1997 and Table 1, each of these classes of targets has been identified by other means, either in T47-D cells Cho et al., 1997 and Tighe et al., in prep or in rat cells using the alternative strategy outlined in Figure 3 Revised Task 7. Therefore we remain confident of the success of this project.
- Genetic Engineering and Molecular Biology
- Medicine and Medical Research