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The Effect of Pure Titanium and Titanium Alloy on Osteoblast and Chondrocyte Maturatin In Vitro.
TEXAS UNIV MEDICAL SCHOOL AT SAN ANTONIO
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Implant success is dependent on the interaction of the implant with surrounding tissues. By understanding this, better devices can be developed. Substrate specific information regarding implant surface characteristics and its influence on mesenchymal cell proliferation and differentiation may be helpful in the design of implants to optimize bone growth at the interface. Based on in vivo and in vitro studies, biomaterial composition, surface energy, surface roughness, and surface topography appear to play a major role in implant success. However, in-depth analysis of cellular response to various implant surface characteristics and the ensuing local and systemic factor production remain to be performed. The first aim of this study was to evaluate the ability of two implant characteristics, chemical composition and surface roughness, to modulate various parameters of osteoblast metabolism in vitro. Commercially pure titanium Ti and titanium-6 wt. aluminum-4 wt. vanadium alloy Ti-A disks were machined to achieve a specific roughness through either fine-polishing S, smooth, Ra 0.2 micronmeter or wet sand-grinding R, rough, Ra 3.0 micronmeter. Profilometry and scanning electron microscopy SEM were used to determine surface roughness, Auger spectroscopy and FT-IR were also used to characterize the disks. MG63 osteoblast-like human osteosarcoma cells were seeded at 9300 cellscm2 and cultured to confluence on standard tissue culture polystyrene or the Ti surfaces. Cell morphology was assessed by SEM. Osteoblast cultures were examined for differences in proliferation as measured by cell number, and 3H-thymidine incorporation differentiation by cell and cell layer alkaline phosphatase specific activity ALpase and osteocalcin production matrix synthesis by proteoglycan sulfation and collagen production and cytokine production by transforming growth factor-Beta and prostaglandin E2 production.
APPROVED FOR PUBLIC RELEASE