The Role of Phospholipase A2 and the Products of Its Action in the Regulation of Chondrocytes by Vitamin D3 Metabolites.
TEXAS UNIV HEALTH SCIENCE CENTER AT SANANTONIO
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Long hone formation, osseous repair, and potentially, guided tissue regeneration, all occur through endochondral bone formation, a process which involves chondrocyte differentiation, proliferation, hypertrophy, and mineralization Vitamin D3 metabolites have been shown to play a pivotal role in chondrocyte differentiation and are therefore essential for proper endochondral classification. In fact, the actions of the vitamin D3 metabolites are cell maturation-dependent with l,25-OH2D3 primarily affecting growth zone chondrocytes and 24,25-OH2D3 primarily affecting resting zone chondrocytes. Prior studies have demonstrated that 1,25-OH2D3 increases phospholipase A2 activity in growth zone chondrocytes, while 24,25-OH2D3 decreases phospholipase A2 activity in resting zone chondrocytes. In addition, these vitamin D3 metabolites mediate their effects in a cell maturation-dependent manner through activation of protein kinase C PKC, an enzyme involved in cellular signal transduction. The aim of the present study was to examine the role of phospholipase A2 in mediating the activation of PKC by vitamin D3 metabolites.
- Anatomy and Physiology
- Medicine and Medical Research