Molecular Basis of P-Nitrophenol (PNP) Biodegradation and its Application in the Environment.
Final rept. 30 Sep 97-29 Sep 98,
INSTITUTE OF MICROBIAL TECHNOLOGY CHANDIGARH (INDIA)
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By taking plasmid samples from the US and India, basic microbial science was related to field application. The distribution of plasmid ecology and gene sequences for a variety of isolates were studied towards an understanding of the genetics and distributions of microorganisms capable of utilizing PNP as their sole source of carbon, nitrogen, and energy. The studies indicate that 1 the degradation of PNP by several organisms proceeds through an oxidative route, as indicated by the accumulation of nitrite molecules in his cultures, 2 the plasmid responsible for carrying the gene for PNP degradation in certain microbes consists of approximately 50 kilobase pairs, and 3 the same plasmid also encodes resistance to inorganiz zinc ions. Genes were successfully cloned as a basis for further environmental application. As a means of natural attenuation, the development of bioremediation technologies could save millions in addressing contamination sites, with wide application to the remediation of contaminants in soil and groundwater everywhere.
- Organic Chemistry