Wnt-5a and Wnt-4 Regulates Cell Growth in C57MG Mammary Epithelial Cells
Final rept. 1 Jul 94-30 Jun 98
PENNSYLVANIA STATE UNIV UNIVERSITY PARK
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The implications for determining whether wnt-5a and wnt-4 act as mediators of normal cell growth are relevant to the detection, diagnosis, and the treatment of breast cancer. That is, it is important ultimately to understand whether the inappropriate down regulation of certain wnt-genes that are spatially-temporally expressed in developing mammary glands, such as wnt-5a and wnt-4, leads to loss of growth control. C57MG mammary epithelial cells have recently been found to endogenously express wnt-5a and wnt-4 whose RNA levels are reduced in the presence wnt-gene family members which transform this cell line. The intent of this proposal has been to develop new cell lines by transfecting C57MG cells with DNA wnt-5a andor wnt-4 constructs which express antisense or sense RNA to determine the effect on cell transformation will occur.
- Genetic Engineering and Molecular Biology
- Anatomy and Physiology
- Medicine and Medical Research