Inhibition of Breast Cancer Growth by IGFBP-3
Annual rept. 1 Oct 97-30 Sep 98
OREGON HEALTH SCIENCES UNIV PORTLAND
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The proposal of my grant is to investigate the biological significance and mechanism of insulin-like growth factor binding protein-3 IGFBP-3 as well as identification and characterization of the IGFBP-3 receptor in human breast cancer cells. As a second year task, I have successfully screened the IGFBP-3 interacting proteins including putative IGFBP-3 receptor in human breast cancer cells by employing the yeast two-hybrid system. Two cDNA clones matched sequences in the GenBank database 1 Eps8 - epidermal growth factor receptor kinase substrate, and 2 GRP78BiP - glucose regulated stress protein, or human immunoglobulin heavy chain binding protein. The third cDNA, designated clone 4-33, was not identified in the database and represents a novel gene protein. I have thus far concentrated my efforts on this novel cDNA clone 1 synthesis of recombinant human 4-33 protein by use of a Glutathione S-Transferase fusion and 2 generation of polyclonal 4-33 antibodies.
- Genetic Engineering and Molecular Biology
- Anatomy and Physiology
- Medicine and Medical Research