Identification and Localization of Genes which Restore Senescence in Breast Cancer Cells
Annual rept. 30 Aug 97-29 Aug 98
TEMPLE UNIV PHILADELPHIA PA
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We have identified a gene on chromosome 16 which restores senescence to human and rat mammary tumor cells by functional complementation. Initial efforts at mapping, using PCR based analysis of rat revertant clones arising from senescent colonies, revealed that the senescence gene was located within a 3-7 cM consensus deletion region at 16q24.3. A yeast artificial chromosome YAC contig of this consensus deletion region was obtained and analyzed for marker content. Two overlapping YACs, 792 E-1 and 624 H-7, spanning this region were retrofitted with a selectable marker. Transfer of these two YACs by spheroplast fusion restored senescence to immortal rat ovarian tumor cells. Indirect transfer of retrofitted YAC 792-E-1 by microcell mediated chromosome transfer restored senescence to immortal human and rat mammary tumor cells.
- Genetic Engineering and Molecular Biology
- Anatomy and Physiology
- Medicine and Medical Research