Puberty Differentiation and Breast Cancer Risk
Annual rept. 15 Sep 97-14 Sep 98
PENNSYLVANIA UNIV PHILADELPHIA
Pagination or Media Count:
BRCA2 is a tumor suppressor gene which has been implicated in a large number of inherited breast cancers. We have generated a full-length human BRCA2 cDNA clone which incorporates silent mutations creating unique restriction sites. Furthermore, we describe a rabbit polyclonal antisernm raised against murine Brca2 which is suitable for both Western blotting and immunoprecipitation. We demonstrate that Brca2 is a nuclear phosphoprotein in a murine mammary epithelial cell line NMuMG , and we demonstrate that protein levels are regulated in multiple mouse cell lines during cell cycle progression. Additionally, we describe progress and plans in creating cell lines which inducibly alter Brca2 levels, and we anticipate using these cell lines to investigate the role of Brca2 in the cellular response to DNA damage and in cell cycle progression. Finally, we describe additional training opportunities which form an integral part of the nature of this grant.
- Anatomy and Physiology
- Medicine and Medical Research